CH25H antibody (AA 1-100)

Catalog No. ABIN1387938

Cited in 2+ publications. The Rabbit Polyclonal anti-CH25H antibody (ABIN1387938) specifically detects CH25H in WB, IF, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CH25H antibody (AA 1-100) (ABIN1387938)

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CH25H antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CH25H Antibody

Binding Specificity: AA 1-100

Purpose: CH25H Polyclonal Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CH25H

Isotype: IgG

Application Details

Application Notes: WB(WB=1:500-2000), IHC-P(IHC-P=1:400-800), IHC-F(IHC-F=1:400-800), IF(IF=1:100-500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CH25H antibody (ABIN1387938)

Sun, Garnier, Chevalier, Roumain, Wang, Angelillo, Montorfani, Pick, Brighouse, Fournier, Tarussio, Tissot, Lobaccaro, Petrova, Jandus, Speiser, Kopf, Pot, Scheiermann, Homicsko, Muccioli, Garg, Hugues: "Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma." in: Nature communications, Vol. 16, Issue 1, pp. 1217, (2025) (PubMed).

Mukherjee, Hough, Chattopadhyay, Navab, Fogelman, Meriwether, Williams, Bensinger, Moller, Faull, Lusis, Iruela-Arispe, Bostrom, Tontonoz, Reddy, Fogelman: "Transgenic tomatoes expressing the 6F peptide and ezetimibe prevent diet-induced increases of IFN-β and cholesterol 25-hydroxylase in jejunum." in: Journal of lipid research, Vol. 58, Issue 8, pp. 1636-1647, (2017) (PubMed).

Target Details for CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Alternative Name: CH25H

Background: Synonyms: C25H, Cholesterol 25 hydroxylase, Cholesterol 25-hydroxylase, Cholesterol 25 monooxygenase, EC 1.14.99.38, EC=1.14.99.38, h25OH, CH25H_HUMAN.

Gene ID: 9023

UniProt: O95992