AP2M1 antibody (AA 201-300) (AbBy Fluor® 350)

Catalog No. ABIN1390107

The AbBy Fluor® 350-conjugated Rabbit Polyclonal anti-AP2M1 antibody (ABIN1390107) specifically detects AP2M1 in IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for AP2M1 antibody (AA 201-300) (AbBy Fluor® 350) (ABIN1390107)

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2M1 antibody is conjugated to AbBy Fluor® 350

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-AP2M1 Antibody (AbBy Fluor® 350)

Binding Specificity: AA 201-300

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AP2M1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AP2M1

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Alternative Name: AP2M1

Background:

Synonyms: Adaptin mu 1, Clathrin coat associated protein AP50, Adapter-related protein complex 2 mu subunit, Adaptin-mu2, Adaptor protein complex AP 2 subunit mu, Adaptor protein complex AP-2 subunit mu, Adaptor related protein complex 2 mu 1 subunit, AP 2 mu 2 chain, AP-2 complex subunit mu, AP-2 mu chain, Ap2m1, AP2M1_HUMAN, AP50, CLAPM1, Clathrin adaptor complex AP2 mu subunit, Clathrin assembly protein complex 2 medium chain, Clathrin associated/assembly/adaptor protein medium 1, Clathrin coat adaptor protein AP50, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, HA2 50 kDa subunit, mu2 antibody, Plasma membrane adaptor AP-2 50 kDa protein.

Background: Adaptins are heterotetrameric subunits of adaptors, which are complexes involved in the formation of Clathrin-coated pits for vesicle-mediated endocytosis. Clathrin and its associated heterotetrameric protein complexes make up the main protein components of the coat surrounding the cytoplasmic face of coated vesicles. The Adaptin family, comprising a, b, and g classes, is also responsible for the transport of ligand-receptor complexes from plasma membranes and the trans-Golgi network to lysosomes. Two main types of adaptor proteins (APs), AP-1 and AP-2, are found in Clathrin-coated structures located at the Golgi complex and the plasma membrane of mammalian cells, respectively. Adaptor protein complex 2 (AP-2) is composed of two large Adaptins (a1A/AP2A1 and b1/AP2B1), a medium Adaptin (m2/AP-2m1) and a small Adaptin (s2 long/AP2S1). AP-2m1, a 435 amino acid protein, links Clathrin to receptors in coated vesicles.

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome