MAPK7 antibody (pThr219, pTyr221) (FITC)

Catalog No. ABIN1391954

The Rabbit Polyclonal anti-MAPK7 antibody has been validated for IF (cc), IF (p) and FACS. It is suitable to detect MAPK7 in samples from Mouse.

Quick Overview for MAPK7 antibody (pThr219, pTyr221) (FITC) (ABIN1391954)

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK7 antibody is conjugated to FITC

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Product Details anti-MAPK7 Antibody (FITC)

Binding Specificity: pThr219, pTyr221

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ERK5 around the phosphorylation site of Thr219 + Tyr221

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MAPK7

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Alternative Name: ERK5 +

Background:

Synonyms: ERK5 phospho Thr219 + Tyr221, ERK5 phospho Thr219 + Tyr221, Big MAP kinase 1, BMK 1, BMK 1 kinase, BMK-1, BMK1, BMK1 Kinase, EC 2.7.11.24, ERK 4, ERK 5, ERK-5, ERK4, ERK5, Extracellular Signal Regulated Kinase 5, Extracellular signal-regulated kinase 5, MAP kinase 7, MAPK 7, MAPK7, Mitogen Activated Protein Kinase 7, Mitogen-activated protein kinase 7, MK07_HUMAN,

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling