PICALM antibody (AA 251-350) (Biotin)

Catalog No. ABIN1392613

This Rabbit Polyclonal antibody specifically detects PICALM in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for PICALM antibody (AA 251-350) (Biotin) (ABIN1392613)

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PICALM antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PICALM Antibody (Biotin)

Binding Specificity: AA 251-350

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Horse,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PICALM/CALM

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for PICALM

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Alternative Name: PICALM/CALM

Background:

Synonyms: Clathrin assembly lymphoid myeloid leukemia, Clathrin assembly lymphoid myeloid leukemia protein, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein, PICAL_HUMAN, PICALM.

Background: Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of clathrin binding adaptin proteins and two smaller subunits known as AP50 and AP17. The Alpha- and Beta-Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. Alpha-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP180 (also designated AP-3 or F1-20) is a synapse-specific clathrin assembly protein. The protein CALM (clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in clathrin assembly.

Pathways: Synaptic Membrane