PICALM antibody (AA 251-350) (Biotin)

Catalog No. ABIN1392613

Product Details anti-PICALM Antibody (Biotin)

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Binding Specificity: AA 251-350

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PICALM antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Horse,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PICALM/CALM

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for PICALM

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Alternative Name: PICALM/CALM (PICALM Products)

Synonyms: LAP-3 antibody, PEPS antibody, CALM antibody, CLTH antibody, LAP antibody, PAP180 antibody, fit-1 antibody, fit1 antibody, mKIAA4114 antibody, Calm antibody, leucine aminopeptidase 3 antibody, phosphatidylinositol binding clathrin assembly protein antibody, si:ch211-200p22.4 antibody, LAP3 antibody, PICALM antibody, Picalm antibody, si:ch211-200p22.4 antibody

Background:

Synonyms: Clathrin assembly lymphoid myeloid leukemia, Clathrin assembly lymphoid myeloid leukemia protein, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein, PICAL_HUMAN, PICALM.

Background: Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of clathrin binding adaptin proteins and two smaller subunits known as AP50 and AP17. The Alpha- and Beta-Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. Alpha-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP180 (also designated AP-3 or F1-20) is a synapse-specific clathrin assembly protein. The protein CALM (clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in clathrin assembly.

Pathways: Synaptic Membrane