Aspartate beta Hydroxylase antibody (AA 301-400) (Biotin)

Catalog No. ABIN1395379

This Rabbit Polyclonal antibody specifically detects Aspartate beta Hydroxylase in ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Aspartate beta Hydroxylase antibody (AA 301-400) (Biotin) (ABIN1395379)

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aspartate beta Hydroxylase antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Aspartate beta Hydroxylase Antibody (Biotin)

Binding Specificity: AA 301-400

Predicted Reactivity: Human,Mouse,Rat,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ASPH/Aspartate beta hydroxylase

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Aspartate beta Hydroxylase

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Alternative Name: ASPH/Aspartate beta hydroxylase

Background:

Synonyms: ASP beta hydroxylase, Aspartyl/asparaginyl beta hydroxylase, ASPH, BAH, CASQ2BP1, HAAH, JCTN, junctin, Peptide aspartate beta dioxygenase, ASPH_HUMAN.

Background: Aspartyl/asparaginyl beta-hydroxylase (ASPH) is a widely-expressed type II membrane protein involved in calcium homeostasis. Located in the endoplasmic reticulum, ASPH specifically hydroxylates an Asp or Asn residue in the epidermal growth factor-like (EGF) domains of several proteins, using iron as a cofactor. The ASPH gene encodes 3 proteins, ASPH, Junctin, and Junctate (or Humbug), that differ significantly in their C-terminal domains. These ASPH gene products are expressed as five transcript variants that differ by their roles in calcium storage and release, hydroxylation capabilities, and tissue specificity. While all ASPH variants are expressed in skeletal muscle, only some are detected in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. In the lumen of the endoplasmic reticulum, ASPH can be processed into two different forms.

Pathways: Positive Regulation of Endopeptidase Activity