MAP1LC3A/B antibody (AA 25-121) (Biotin)

Catalog No. ABIN1396212

This anti-MAP1LC3A/B antibody is a Rabbit Polyclonal antibody detecting MAP1LC3A/B in IHC (p) and ELISA. Suitable for Human and Rat.

Quick Overview for MAP1LC3A/B antibody (AA 25-121) (Biotin) (ABIN1396212)

Target: MAP1LC3A/B (MAP1LC3A,B)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A/B antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA

Product Details anti-MAP1LC3A/B Antibody (Biotin)

Binding Specificity: AA 25-121

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MAP LC3 Alpha

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MAP1LC3A/B

Target: MAP1LC3A/B (MAP1LC3A,B)

Alternative Name: MAP 1A/1B LC3 Alpha/Beta

Background:

Synonyms: Microtubule-associated proteins 1A/B light chain 3A, Microtubule-associated proteins 1A/B light chain 3B, Microtubule-associated proteins 1Beta light chain 3A, Microtubule-associated proteins 1Beta light chain 3A, MAP1B LC3 A, MAP LC3 Beta, MAP-LC3 Beta, MAP1 light chain 3-like protein 1.MAP1LC3B.MLP3A_HUMAN

Background: Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3 alpha) are subunits of both MAP1A and MAP1B. MAP-LC3M Beta, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3 Beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3? untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3 Beta may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Gene ID: 54901, 84557

UniProt: Q9H492, Q9GZQ8