Influenza Nucleoprotein antibody (Influenza A Virus (A/Russia:St.Petersburg/8/2006)) (AA 71-170) (Biotin)

Catalog No. ABIN1397196

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, IHC (fro) and IHC (p). Suitable for Influenza A Virus and Virus. This Primary Antibody has been cited in 1 publication.

Quick Overview for Influenza Nucleoprotein antibody (Influenza A Virus (A/Russia:St.Petersburg/8/2006)) (AA 71-170) (Biotin) (ABIN1397196)

Target: Influenza Nucleoprotein (NP)

Reactivity: Influenza A Virus, Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 71-170

Virus Strain: A/Russia:St.Petersburg/8/2006

Cross-Reactivity: Virus

Cross-Reactivity (Details): Influenza A virus

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Influenza A virus (strain A/Russia:St.Petersburg/8/2006 H1N1) Nucleoprotein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References

Temple, Finger, Jones, Gabbard, Jelesijevic, Uhl, Hogan, Glenn, Tompkins: "IN OVO AND IN VITRO SUSCEPTIBILITY OF AMERICAN ALLIGATORS (ALLIGATOR MISSISSIPPIENSIS) TO AVIAN INFLUENZA VIRUS INFECTION." in: Journal of wildlife diseases, (2014) (PubMed).

Target Details

Target: Influenza Nucleoprotein (NP)

Alternative Name: Influenza A virus Nucleoprotein

Target Type: Influenza Protein

Background:

Synonyms: Nucleoprotein, NP, Nucleocapsid protein, Protein N, Influenza A virus H1N1, H3N2 Nucleoprotein, H9N2 Nucleoprotein, H2N2 Nucleoprotein, H3N8 Nucleoprotein, H7N7 Nucleoprotein, H5N1 Nucleoprotein.

Background: Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.