Influenza Nucleoprotein antibody (Influenza A Virus H2N2) (H1N1), (H2N2), (H3N2), (H5N1), (H5N2) (HRP)

Catalog No. ABIN2452038

Product Details

Target: Influenza Nucleoprotein (NP)

Binding Specificity: H1N1, H2N2, H3N2, H5N1, H5N2

Reactivity: Influenza A Virus H2N2

Host: Mouse

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC)

Specificity: Reacts with NP of all influenza A viruses tested so far, including seasonal H2N2, H3N2, and avian H5N1, H5N2 and H1N1 (seasonal, pandemic and swine).

Cross-Reactivity (Details): No cross reactivity with influenza B viruses.

Purification: Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions.

Sterility: Sterile filtered

Immunogen: Human Influenza A Virus (H2N2) Okada strain

Clone: C43

Isotype: IgG2a

Application Details

Application Notes: 1) Western blotting: 300~1,000 fold dilution
2) Immunocytochemistry: ~200 fold dilution
4) Immunohistochemistry: ~200 fold dilution
5) ELISA (assay dependent)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References

Mizuike, Sasaki, Baba, Iwamoto, Shibai, Kosaka, Kubota-Koketsu, Yang, Du, Sakudo, Tsujikawa, Yunoki, Ikuta: "Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1." in: Clinical and vaccine immunology : CVI, Vol. 18, Issue 3, pp. 494-9, (2011) (PubMed).

Ueda, Yamate, Du, Daidoji, Okuno, Ikuta, Nakaya: "Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus." in: Virus research, Vol. 136, Issue 1-2, pp. 91-7, (2008) (PubMed).

Okuno, Isegawa, Sasao, Ueda: "A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains." in: Journal of virology, Vol. 67, Issue 5, pp. 2552-8, (1993) (PubMed).

Target Details

Target: Influenza Nucleoprotein (NP)

Alternative Name: Influenza A (Nucleoprotein) (NP Products)

Synonyms: nucleoprotein antibody, NP antibody

Target Type: Influenza Protein

Background: Influenza virus is an RNA virus, which causes influenza, and belongs to the family Orthomyxoviridae. Influenza virus is classified into three different genera, influenzavirus A, B, and C. They all have similar structures and compositions. The virions are 80-100nm in diameter and usually roughly spherical. The outer surface of the virion is made of a viral envelope containing two major glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Influenzavirus A is further classified into subtypes based on the surface glycoproteins, HA and NA. Currently, there are 16 HA and 9 NA subtypes. The central core of the virion contains the viral RNA genome, which is packaged in the form of ribonucleoprotein complexes. Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly expressed during the course of infection. It is a structural protein, which encapsidates the negative strand viral RNA and is essential for RNA transcription, replication and packaging. NP binds the PB1 and PB2 subunits of the viral RNA polymerase and the matrix protein M1, in addition to its binding to ssRNA. NP is also known to interact with variety of other macromolecules of both viral and cellular origins, and these interactions have been shown to be essential for the viral lifecycle.