Nucleolin antibody (AA 601-710) (AbBy Fluor® 555)

Catalog No. ABIN1399589

The Rabbit Polyclonal anti-Nucleolin antibody has been validated for FACS, IF (p) and IF (cc). It is suitable to detect Nucleolin in samples from Human and Rat.

Quick Overview for Nucleolin antibody (AA 601-710) (AbBy Fluor® 555) (ABIN1399589)

Target: Nucleolin (NCL)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is conjugated to AbBy Fluor® 555

Application: Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Nucleolin Antibody (AbBy Fluor® 555)

Binding Specificity: AA 601-710

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nucleolin

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: C23

Background:

Synonyms: C23, Nucleolin, Protein C23, NCL

Background: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization