Nitrotyrosine antibody (AbBy Fluor® 555)

Catalog No. ABIN1399673

The Rabbit Polyclonal anti-Nitrotyrosine antibody has been validated for WB, FACS, IF (p) and IF (cc). It is suitable to detect Nitrotyrosine in samples from Human, Mouse and Rat.

Quick Overview for Nitrotyrosine antibody (AbBy Fluor® 555) (ABIN1399673)

Target: Nitrotyrosine

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nitrotyrosine antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Nitrotyrosine Antibody (AbBy Fluor® 555)

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Nitrotyrosine

Purification: Purified by Protein A.

Immunogen: KLH conjugated to Nitrotyrosine

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical

Background:

Synonyms: NULL

Background: Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.