BRD4 antibody (AbBy Fluor® 488)

Catalog No. ABIN1404496

This Rabbit Polyclonal antibody specifically detects BRD4 in WB and IF (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BRD4 antibody (AbBy Fluor® 488) (ABIN1404496)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-BRD4 Antibody (AbBy Fluor® 488)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Brd4/CAP

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4/CAP

Background:

Synonyms: Brd4, BRD4_HUMAN, bromodomain containing protein 4, Bromodomain-containing protein 4, CAP, chromosome associated protein, HUNK1, Protein HUNK1.

Background: Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G(1) to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome.

Gene ID: 23476

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome