PDGFA antibody (AA 102-213) (HRP)

Catalog No. ABIN1406366

The Rabbit Polyclonal anti-PDGFA antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect PDGFA in samples from Human and Mouse.

Quick Overview for PDGFA antibody (AA 102-213) (HRP) (ABIN1406366)

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFA antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PDGFA Antibody (HRP)

Binding Specificity: AA 102-213

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rabbit PDGF-A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDGFA

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Alternative Name: PDGF A

Background:

Synonyms: PDGFA, PDGF AA, PDGF-AA, PDGF A chain, PDGF subunit A, PDGF-1, PDGF1, PDGFA, PDGFA_HUMAN, Platelet derived growth factor A chain precursor, Platelet derived growth factor alpha polypeptide, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Platelet-derived growth factor subunit A.

Background: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. The long form contains a basic insert which acts as a cell retention signal. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. It belongs to the PDGF/VEGF growth factor family.

Gene ID: 5154

UniProt: P04085

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling