DAB2 antibody (pSer24) (Cy7)

Catalog No. ABIN1406832

This anti-DAB2 antibody is a Rabbit Polyclonal antibody detecting DAB2 in WB, IF (cc) and IF (p). Suitable for Mouse.

Quick Overview for DAB2 antibody (pSer24) (Cy7) (ABIN1406832)

Target: DAB2 (Disabled Homolog 2, Mitogen-Responsive phosphoprotein (Drosophila) (DAB2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAB2 antibody is conjugated to Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DAB2 Antibody (Cy7)

Binding Specificity: pSer24

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human DAB2 around the phosphorylation site of Ser24 [AP(p-S)KK]

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DAB2

Target: DAB2 (Disabled Homolog 2, Mitogen-Responsive phosphoprotein (Drosophila) (DAB2))

Alternative Name: DAB2

Background:

Synonyms: DOC2, DOC-2, Disabled homolog 2, Adaptor molecule disabled-2, Differentially expressed in ovarian carcinoma 2, Differentially-expressed protein 2, DAB2

Background: Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1, this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Involved in CFTR trafficking to the late endosome. Involved in several receptor-mediated signaling pathways. Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2. Mediates TFG-beta-stimulated JNK activation. May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1 (PP1). Sequesters LRP6 towards clathrin-mediated endocytosis, leading to inhibition of Wnt/beta-catenin signaling. May activate non-canonical Wnt signaling.

Gene ID: 1601

UniProt: P98082

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process