PCDHB12 antibody (AA 121-220) (HRP)

Catalog No. ABIN1407482

The Rabbit Polyclonal anti-PCDHB12 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect PCDHB12 in samples from Human.

Quick Overview for PCDHB12 antibody (AA 121-220) (HRP) (ABIN1407482)

Target: PCDHB12 (Protocadherin beta 12 (PCDHB12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCDHB12 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PCDHB12 Antibody (HRP)

Binding Specificity: AA 121-220

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCDHB12

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PCDHB12

Target: PCDHB12 (Protocadherin beta 12 (PCDHB12))

Alternative Name: PCDHB12

Background:

Synonyms: PCDH beta12, PCDHB 12, PCDHB12, PCDBC_HUMAN.

Background: Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHB12 (Protocadherin beta 12) is a 795 amino acid protein that is one of 16 proteins in the protocadherin beta cluster. Unlike the alpha and gamma gene clusters whose genes are spliced to downstream constant region exons during transcription, members of the beta cluster (such as PCDHB12) do not use constant-region exons to produce mRNAs. As a result, each protocadherin beta gene encodes the transmembrane, extracellular and short cytoplasmic domains of the protein. Localized to the cell membrane, PCDHB12 is a single-pass type I membrane protein that contains six cadherin domains. PCDHB12 may participate in the establishment and maintenance of specific neuronal connections in the brain.