SUFUH antibody (AA 433-484) (Cy5)

Catalog No. ABIN1407808

This Rabbit Polyclonal antibody specifically detects SUFUH in WB, IF (cc) and IF (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SUFUH antibody (AA 433-484) (Cy5) (ABIN1407808)

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUFUH antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SUFUH Antibody (Cy5)

Binding Specificity: AA 433-484

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SUFU

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SUFUH

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Alternative Name: SUFU/Suppressor of Fused

Background:

Synonyms: SUFUH, SUFUXL, PRO1280, Suppressor of fused homolog, SUFU, UNQ650/PRO1280

Background: Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity).

Gene ID: 51684

UniProt: Q9UMX1

Pathways: Hedgehog Signaling, Tube Formation, Maintenance of Protein Location