SUFUH antibody (N-Term)

Catalog No. ABIN6265364

Product Details anti-SUFUH Antibody

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUFUH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: SUFU Antibody detects endogenous levels of total SUFU.

Predicted Reactivity: Bovine,Horse,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SUFU, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SUFUH

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Alternative Name: SUFU (SUFUH Products)

Synonyms: CG6054 antibody, Dmel\\CG6054 antibody, SU(FU) antibody, SUFU antibody, Su(Fu) antibody, SuFu antibody, Sufu antibody, su(fu) antibody, sufu antibody, Xsufu antibody, sufuh antibody, sufuxl antibody, PRO1280 antibody, SUFUH antibody, SUFUXL antibody, Su(fu) antibody, dre antibody, fi39e05 antibody, wu:fi39e05 antibody, 2810026F04Rik antibody, b2b273Clo antibody, Suppressor of fused antibody, SUFU negative regulator of hedgehog signaling L homeolog antibody, SUFU negative regulator of hedgehog signaling antibody, suppressor of fused homolog (Drosophila) antibody, Su(fu) antibody, sufu.L antibody, SUFU antibody, sufu antibody, Sufu antibody

Background:

Description: Negative regulator in the hedgehog/smoothened signaling pathway. Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24311597, PubMed:24217340). Down-regulates GLI2-mediated transactivation of target genes (PubMed:24311597, PubMed:24217340). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10806483, PubMed:24217340). Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development. Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity).

Gene: SUFU

Molecular Weight: 54 kDa

Gene ID: 51684

UniProt: Q9UMX1

Pathways: Hedgehog Signaling, Tube Formation, Maintenance of Protein Location