Endothelin 2 antibody (AA 49-59) (HRP)

Catalog No. ABIN1408226

The Rabbit Polyclonal anti-Endothelin 2 antibody has been validated for ELISA, IHC (fro) and IHC (p). It is suitable to detect Endothelin 2 in samples from Cat.

Quick Overview for Endothelin 2 antibody (AA 49-59) (HRP) (ABIN1408226)

Target: Endothelin 2 (EDN2)

Reactivity: Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endothelin 2 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Endothelin 2 Antibody (HRP)

Binding Specificity: AA 49-59

Cross-Reactivity: Cat

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Cat,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Endolin 2

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Endothelin 2

Target: Endothelin 2 (EDN2)

Alternative Name: ET-2/Endothelin 2

Background:

Synonyms: EDN2, EDN2_HUMAN, Endothelin 2, Endothelin-2, ET 2, ET-2, ET2, PPET2, Preproendothelin 2, Preproendothelin-2.

Background: The endothelin (ET) family of proteins, which includes ET-1 (endothelin-1), ET-2 (endothelin-2) and ET-3 (endothelin-3), are vasoactive peptides that are involved in various functions throughout the body. Endothelins can affect the central nervous system and neuronal excitability, and they elicit potent vasoconstrictor action. While ET-1 is a potent, 21-amino acid vasoconstrictor peptide, ET-2 has the most potent vasoconstrictor activity. ET-3 functions as a ligand for endothelin receptor type B (ETBR) and, through this interaction, mediates the maturation of enteric neurons and melanocytes. Although ET-3 is expressed as a 238 amino acid peptide, it is post-translationally modified to produce a short active isoform and a long inactive isoform. Defects in the gene encoding ET-3 are the cause of a variety of disorders, including Hirschsprung disease type 1 (HSCR1), congenital central hypoventilation syndrome (CCHS) and Waardenburg syndrome type IV (WS4).

Pathways: Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones