IFT57 antibody (AA 331-429) (Cy5.5)

Catalog No. ABIN1410695

The Rabbit Polyclonal anti-IFT57 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect IFT57 in samples from Human, Mouse and Rat.

Quick Overview for IFT57 antibody (AA 331-429) (Cy5.5) (ABIN1410695)

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT57 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-IFT57 Antibody (Cy5.5)

Binding Specificity: AA 331-429

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIPPI

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IFT57

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Alternative Name: HIPPI

Background:

Synonyms: Dermal papilla derived protein 8, Dermal papilla-derived protein 8, DERP 8, DERP8, Esrrbl 1, Esrrbl1, ESRRBL1 protein, Estrogen related receptor beta like 1, Estrogen related receptor beta like protein 1, Estrogen-related receptor beta like 1, Estrogen-related receptor beta-like protein 1, FLJ10147, Hip1 Interacting Protein, HIP1 interacting protein HIPPI, Hip1 protein interactor, HIP1-interacting protein, HIPPI, Huntingtin interacting protein 1 interacting protein, Huntingtin interacting protein 1 protein interactor, Huntingtin-interacting protein-1 protein interactor, T 57, t57, T57_HUMAN, Intraflagellar transport 57 homolog Chlamydomonas, Intraflagellar transport 57 homolog, Intraflagellar transport protein 57 homolog, MHS4R2, Vestrogen related receptor beta like 1, Vestrogen-related receptor beta like 1, CDNA FLJ10147 fis clone HEMBA1003369.

Background: Programmed neuronal cell death is a feature of neurodegenerative disorders such as Alzheimer's and Huntington's disease, which occur later in human life. Huntington?s disease at the molecular and cell level is characterized by polyglutamine expansion of the protein huntingtin (Htt) that leads to apoptotis-mediated neurodegenerative loss of medium spiny neurons throughout the striatum. Polyglutamine expansion reduces the level of association between Hip-1 and Htt, thereby increasing levels of free Hip-1 that then can be the candidate protein Hippi (Hip-1 protein interactor). The Hippi-Hip-1 heterodimer is a pro-apoptotic complex that recruits procaspase-8 and initiates caspase-8 activation, which may contribute to the neuronal cell death observed in individuals diagnosed with Huntington?s disease. The human hippi gene maps to chromosome 3q13.13 and encodes a 429 amino acid protein.

Pathways: Hedgehog Signaling, Positive Regulation of Endopeptidase Activity