IFT57 antibody (Internal Region)

Catalog No. ABIN361194

The Goat Polyclonal anti-IFT57 antibody (ABIN361194) specifically detects IFT57 in WB, IHC and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for IFT57 antibody (Internal Region) (ABIN361194)

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT57 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-IFT57 Antibody

Binding Specificity: Internal Region

Purpose: HIPPI

Sequence: EQPQEYDDPN ATISN

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQPQEYDDPNATISN , from the internal region of the protein sequence according to NP_060480.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 49 kDa band observed in Human and Mouse Brain lysates (calculated MW of 49.1 kDa according to Human NP_060480.1 and of 48.8 kDa according to Mouse NP_082956.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IFT57

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Alternative Name: IFT57

Background: IFT57, intraflagellar transport 57 homolog (Chlamydomonas), ESRRBL1, FLJ10147, HIPPI, MHS4R2, HIP1 protein interactor, estrogen-related receptor beta like 1, huntingtin interacting protein-1 interacting protein

Gene ID: 55081, 73916, 303968

NCBI Accession: NP_060480

Pathways: Hedgehog Signaling, Positive Regulation of Endopeptidase Activity