HCV NS4b antibody (AA 1712-1820) (HRP)

Catalog No. ABIN1414689

Product Details anti-HCV NS4b Antibody (HRP)

Target: HCV NS4b (Hepatitis C Virus NS4b (HCV NS4b))

Binding Specificity: AA 1712-1820

Reactivity: Hepatitis C Virus (HCV), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCV NS4b antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Virus

Cross-Reactivity (Details): HCV

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Hepatitis C Virus NS4B

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HCV NS4b

Target: HCV NS4b (Hepatitis C Virus NS4b (HCV NS4b))

Alternative Name: Hepatitis C Virus NS4B (HCV NS4b Products)

Target Type: Viral Protein

Background:

Synonyms: HCV, HCV NS4B, Non structural protein 4B, NS4B, p27.

Background: HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. NS4B induces a specific membrane alteration that serves as a scaffold for the virus replication complex. This membrane alteration gives rise to the so-called ER-derived membranous web that contains the replication complex.

UniProt: P26663