DHX32 antibody (AA 561-660) (Cy5)

Catalog No. ABIN1415489

The Rabbit Polyclonal anti-DHX32 antibody is suitable to detect DHX32 in samples from Human. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for DHX32 antibody (AA 561-660) (Cy5) (ABIN1415489)

Target: DHX32 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 32 (DHX32))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX32 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DHX32 Antibody (Cy5)

Binding Specificity: AA 561-660

Purpose: DHX32 Polyclonal Antibody, Cy5 Conjugated

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DHX32

Isotype: IgG

Application Details

Application Notes: WB(1:500-1000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DHX32

Target: DHX32 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 32 (DHX32))

Alternative Name: DHX32

Background:

Synonyms: Ddx32, DEAD/H Asp-Glu-Ala-Asp/His box polypeptide 32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH Asp-Glu-Ala-His box polypeptide 32, DEAH box protein 32, DHLP1, DHX32, Helicase DDX32, HuDDX32, Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, DHX32_HUMAN.

Background: DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX32 is a 743 amino acid nuclear protein that localizes to the mitochondria and is a member of the DEAD box helicase family. Expressed in various tissues, DDX32 is up-regulated by ionomycin in T lymphocytes and down-regulated in acute lymphoblastic leukemia. Considered a novel RNA helicase, DDX32 may play an important role in the development of colorectal cancer and may be involved in regulating T-cell response to certain apoptotic stimuli.