DHX32 antibody (AA 561-660) (HRP)

Catalog No. ABIN1415493

Product Details anti-DHX32 Antibody (HRP)

Target: DHX32 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 32 (DHX32))

Binding Specificity: AA 561-660

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX32 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DHX32

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DHX32

Target: DHX32 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 32 (DHX32))

Alternative Name: DHX32 (DHX32 Products)

Synonyms: DDX32 antibody, DHLP1 antibody, 3110079L04Rik antibody, 4732469F02Rik antibody, AA408140 antibody, Ddx32 antibody, DEAH (Asp-Glu-Ala-His) box polypeptide 32 antibody, DEAH (Asp-Glu-Ala-His) box polypeptide 32b antibody, DEAH-box helicase 32 (putative) antibody, DEAH-box helicase 32 (putative) L homeolog antibody, DHX32 antibody, dhx32b antibody, Dhx32 antibody, dhx32.L antibody

Background:

Synonyms: Ddx32, DEAD/H Asp-Glu-Ala-Asp/His box polypeptide 32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH Asp-Glu-Ala-His box polypeptide 32, DEAH box protein 32, DHLP1, DHX32, Helicase DDX32, HuDDX32, Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, DHX32_HUMAN.

Background: DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX32 is a 743 amino acid nuclear protein that localizes to the mitochondria and is a member of the DEAD box helicase family. Expressed in various tissues, DDX32 is up-regulated by ionomycin in T lymphocytes and down-regulated in acute lymphoblastic leukemia. Considered a novel RNA helicase, DDX32 may play an important role in the development of colorectal cancer and may be involved in regulating T-cell response to certain apoptotic stimuli.