UBE2H antibody (AA 101-183) (HRP)

Catalog No. ABIN1416562

This Rabbit Polyclonal antibody specifically detects UBE2H in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for UBE2H antibody (AA 101-183) (HRP) (ABIN1416562)

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2H antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2H Antibody (HRP)

Binding Specificity: AA 101-183

Predicted Reactivity: Mouse,Rat,Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ube2H/UBCH2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2H

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Alternative Name: Ube2H

Background:

Synonyms: E2 20K, Ubc H2, UBC8, UBCH 2, UBCH, UBCH2, Ube2h, UBE2H_HUMAN, Ubiquitin carrier protein H, Ubiquitin conjugating enzyme E2 H, Ubiquitin conjugating enzyme E2H UBC8 homolog yeast, Ubiquitin conjugating enzyme E2H, Ubiquitin protein ligase H, Ubiquitin-conjugating enzyme E2 H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H.

Background: UBE2H, also known as UBC8, UBCH, UBCH2 or E2-20K, is a 183 amino acid protein involved in ubiquitin-mediated protein degradation. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). One of several members of the ubiquitin-conjugating enzyme family, UBE2H functions as an E2 ubiquitin-conjugating enzyme that acts to catalyze the covalent attachment of ubiquitin residues to various proteins, including Histone H2A. UBE2H shares 100 % identity with its mouse counterpart and 98 % identity with its frog and zebrafish homologs, suggesting a conserved function between species. Multiple isoforms of UBE2H exist due to alternative splicing events.

Gene ID: 7328