FBXL21 antibody (AA 121-220) (HRP)

Catalog No. ABIN1417186

Product Details anti-FBXL21 Antibody (HRP)

Target: FBXL21 (F-Box and Leucine-Rich Repeat Protein 21 (FBXL21))

Binding Specificity: AA 121-220

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXL21 antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FBXL21/FBL3B

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FBXL21

Target: FBXL21 (F-Box and Leucine-Rich Repeat Protein 21 (FBXL21))

Alternative Name: FBXL21 (FBXL21 Products)

Background:

Synonyms: F box and leucine rich repeat protein 21, F box and leucine rich repeat protein 3 pseudogene, F box and leucine rich repeat protein 3B, F box protein Fbl3b, F box/LRR repeat protein 21, F box/LRR repeat protein 3B, Fbl 21, FBXL 21, FBXL-21, FBL 3, FBL 3B, Fbl21, FBL3, FBL-3B, FBL 3B, FBL3B, FBXL 21, FBXL 3B, FBXL 3P, FBXL3B, FBXL3P, MGC120237, FXL21_HUMAN.

Background: FBL3B is a 434 amino acid protein encoded by the human gene FBXL21. FBL3B contains one 40 amino acid F-box region, making it a member of the F-box family. FBL3B also contains three LRR (leucine-rich) repeats. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. F-box proteins are members of a large family that regulates cell cycle, immune response, signaling cascades and developmental programs by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-a and b-catenin, for degradation by the proteasome after ubiquitination. FBL3B is a substrate-recognition component of the SCF complex that interacts with Skp1 p19 and CUL-1. FBL3B is also associated with expression and regulation of circadian and cryptochrome proteins.

Gene ID: 26223

Pathways: Photoperiodism