Dihydrofolate Reductase antibody (Cy3)

Catalog No. ABIN1418459

The Rabbit Polyclonal anti-Dihydrofolate Reductase antibody has been validated for IF (p) and FACS. It is suitable to detect Dihydrofolate Reductase in samples from Human.

Quick Overview for Dihydrofolate Reductase antibody (Cy3) (ABIN1418459)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is conjugated to Cy3

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Product Details anti-Dihydrofolate Reductase Antibody (Cy3)

Purification: purified by Protein A

Immunogen: KLH conjugated synthetic peptide derived from human DHFR

Isotype: IgG

Application Details

Comment:

Excitation / Emission wavelength: 510.550nm / 570.615nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 µg/µL

Buffer: Aqueous buffered solution containing 100 µg/mL BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4 °C for 12 months

Expiry Date: 12 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background: DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Molecular Weight: 21kDa

Gene ID: 1719

HGNC: 0

Pathways: Mitotic G1-G1/S Phases