RLIM antibody (AA 550-600) (Cy5)

Catalog No. ABIN1419150

This anti-RLIM antibody is a Rabbit Polyclonal antibody detecting RLIM in WB and IF (p). Suitable for Human, Mouse and Rat.

Quick Overview for RLIM antibody (AA 550-600) (Cy5) (ABIN1419150)

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RLIM antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-RLIM Antibody (Cy5)

Binding Specificity: AA 550-600

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RNF12

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RLIM

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Alternative Name: RNF12

Background:

Synonyms: RNF12, NY-REN-43, E3 ubiquitin-protein ligase RLIM, LIM domain-interacting RING finger protein, RING finger LIM domain-binding protein, R-LIM, RING finger protein 12, Renal carcinoma antigen NY-REN-43, RLIM

Background: E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.

Gene ID: 51132

UniProt: Q9NVW2