RLIM antibody (C-Term)

Catalog No. ABIN2779480

The Rabbit Polyclonal anti-RLIM antibody is suitable to detect RLIM in samples from Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for RLIM antibody (C-Term) (ABIN2779480)

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RLIM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RLIM Antibody

Binding Specificity: C-Term

Purpose: RNF12 Antibody - C-terminal region

Sequence: AQFFLLNEDD DDQPRGLTKE QIDNLAMRSF GENDALKTCS VCITEYTEGN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%, Yeast: 89%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RNF12. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RNF12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32151-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RLIM

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Alternative Name: RNF12

Background:

Background Information: RNF12 is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported.

Gene Name: Ring finger protein, LIM domain interacting

Alternative Symbols: MRX61, RNF12, TOKAS, NY-REN-43

Protein Name: E3 ubiquitin-protein ligase RLIM

Molecular Weight: 69kDa

Gene ID: 51132

NCBI Accession: NP_057204

UniProt: Q9NVW2