CHAF1A antibody (HRP)

Catalog No. ABIN1420318

This Rabbit Polyclonal antibody specifically detects CHAF1A in WB, IHC (p). It exhibits reactivity toward Human, Mouse, Rat.

Quick Overview for CHAF1A antibody (HRP) (ABIN1420318)

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHAF1A antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHAF1A Antibody (HRP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human p150 CAF1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHAF1A

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Alternative Name: p150 CAF1

Background:

Synonyms: CAF-1 p150, CAF 1 150 kDa subunit, CAF 1, CAF 1 subunit A, CAF, CAF I 150 kDa subunit, CAF I p150, CAF Ip150, CAF-1 subunit A, CAF-I 150 kDa subunit, CAF-I p150, CAF1 150 kDa subunit, CAF1, CAF1 p150 Subunit, CAF1 subunit A, CAF1A, CAF1A, CAF1A_HUMAN, CAF1B, CAF1P150, CAF1P155, CAF1P155, CHAF1A, Chromatin assembly factor 1 subunit A, Chromatin Assembly Factor 1 Subunit A p150, Chromatin assembly factor I 150 kDa, Chromatin assembly factor I 150 kDa, Chromatin Assembly Factor I, Chromatin assembly factor I p150 subunit, DCAF1, hp150, Nucleosome Remodeling Factor 150 kDa Subunit, NURF150, P150.

Background: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Gene ID: 10036