ALAS2 antibody (Cy3)

Catalog No. ABIN1421045

The Rabbit Polyclonal anti-ALAS2 antibody has been validated for WB and IF (p). It is suitable to detect ALAS2 in samples from Human, Mouse and Rat.

Quick Overview for ALAS2 antibody (Cy3) (ABIN1421045)

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS2 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ALAS2 Antibody (Cy3)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ALAS2/ALAS-E

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ALAS2

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Alternative Name: ALAS-E

Background:

Synonyms: 5-aminolevulinate synthase, erythroid-specic, mitochondrial, 5-aminolevulinic acid synthase, ALAS E, ALASE, ANH1, Delta aminolevulinate synthase, XLSA, 5 aminolevulinic acid synthase 2, 5-aminolevulinate synthase 2, 5-aminolevulinate synthase, 5-aminolevulinate synthase 2, Alas 2, ALAS, ALAS E, ALAS, erythroid, ALASE, Aminolevulinate, delta-, synthase 2, Aminolevulinic acid synthase 2, erythroid, ANH1, ASB, Delta ALA synthase 2, Delta ALA synthetase, Delta aminolevulinate synthase 2, Delta aminolevulinate synthase, Erythroid specic ALAS, FLJ93603, XLDPP, XLSA.

Background: 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

Gene ID: 212

Pathways: Transition Metal Ion Homeostasis