GFI1B antibody (Cy3)

Catalog No. ABIN1423079

The Rabbit Polyclonal anti-GFI1B antibody has been validated for WB and IF (p). It is suitable to detect GFI1B in samples from Human, Mouse and Rat.

Quick Overview for GFI1B antibody (Cy3) (ABIN1423079)

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFI1B antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GFI1B Antibody (Cy3)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GFI1B

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GFI1B

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Alternative Name: GFI1B

Background:

Synonyms: Potential regulator of CDKN1A translocated in CML, GFI 1B, GFI-1B, gfi1b, GFI1B protein, GFI1B_HUMAN, Growth factor independent 1B protein, Growth factor independent protein 1B, Potential regulator of CDKN1A, Translocated in CML, Zinc finger protein Gfi-1b.

Background: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Gene ID: 8328

Pathways: Cellular Response to Molecule of Bacterial Origin