GFI1B antibody (N-Term)

Catalog No. ABIN2779273

The Rabbit Polyclonal anti-GFI1B antibody (ABIN2779273) specifically detects GFI1B in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Goat and Pig samples.

Quick Overview for GFI1B antibody (N-Term) (ABIN2779273)

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Goat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFI1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GFI1B Antibody

Binding Specificity: N-Term

Purpose: GFI1B Antibody - N-terminal region

Sequence: MPRSFLVKSK MAHTYHQPRV QEDEPLWPPA LTPVPRDQAP SNSPVLSTLF

Predicted Reactivity: Cow: 83%, Dog: 100%, Goat: 83%, Horse: 100%, Human: 100%, Mouse: 83%, Pig: 93%, Rat: 82%

Characteristics: This is a rabbit polyclonal antibody against GFI1B. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GFI1B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8101951

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GFI1B

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Alternative Name: GFI1B

Background:

Background Information: GFI1B acts in the late stage of erythroid differentiation as a transcriptional repressor. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis.

Gene Name: Growth factor independent 1B transcription repressor

Alternative Symbols: BDPLT17, ZNF163B

Protein Name: Zinc finger protein Gfi-1b

Molecular Weight: 38kDa

Gene ID: 8328

NCBI Accession: NP_004179

UniProt: Q5VTD9

Pathways: Cellular Response to Molecule of Bacterial Origin