GAPDH antibody (C-Term)

Catalog No. ABIN1440020

The Goat Polyclonal anti-GAPDH antibody has been validated for WB, IF, IHC (p) and IHC (fro). It is suitable to detect GAPDH in samples from Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Monkey, Sheep, Cow, Hamster, Cat, Horse, Guinea Pig, Goat, Avian and Donkey. There are 4+ publications available.

Quick Overview for GAPDH antibody (C-Term) (ABIN1440020)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Monkey, Sheep, Cow, Hamster, Cat, Horse, Guinea Pig, Goat, Avian, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Specificity: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Characteristics: Glyceraldehyde-3-phosphate dehydrogenase

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-GAPDH antibody (ABIN1440020)

Khalafalla, Kayani, Kassab, Ilves, Monsanto, Alvarez, Chavarria, Norman, Dembitsky, Sussman: "Empowering human cardiac progenitor cells by P2Y14 nucleotide receptor overexpression." in: The Journal of physiology, Vol. 595, Issue 23, pp. 7135-7148, (2018) (PubMed).

Gonçalves, Lin, Muthusamy, Fontes-Ribeiro, Ambrósio, Abcouwer, Fernandes, Antonetti: "Protective Effect of a GLP-1 Analog on Ischemia-Reperfusion Induced Blood-Retinal Barrier Breakdown and Inflammation." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2584-92, (2017) (PubMed).

Hyenne, Apaydin, Rodriguez, Spiegelhalter, Hoff-Yoessle, Diem, Tak, Lefebvre, Schwab, Goetz, Labouesse: "RAL-1 controls multivesicular body biogenesis and exosome secretion." in: The Journal of cell biology, Vol. 211, Issue 1, pp. 27-37, (2016) (PubMed).

Helsby, Leader, Fenn, Gulsen, Bryant, Doughton, Sharpe, Whitley, Caunt, James, Pope, Kelly, Chalmers: "CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited." in: BMC cell biology, Vol. 15, pp. 6, (2014) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Synonyms: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
Ensembl identifier: ENSG00000111640