mCherry antibody

Catalog No. ABIN1440058

This Goat Polyclonal antibody specifically detects mCherry in WB, IF, IP, IHC (fro), IHC (p) and IEM. It exhibits reactivity toward Discosoma and has been mentioned in 19+ publications.

Quick Overview for mCherry antibody (ABIN1440058)

Target: mCherry (mCherry Fluorescent Protein)

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: This mCherry antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectron Microscopy (IEM)

Product Details anti-mCherry Antibody

Purpose: Goat polyclonal antibody to mCherry

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody recognizes very well tdTomato and does not recognize GFP (green fluorescent protein).

Cross-Reactivity (Details): Red Fluorescent Protein (dsRed), tdTomato and mCherry.

Characteristics: Goat polyclonal antibody to mCherry (Cherry fluorescent protein).

Purification: This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: Western blot: 1:500-1:5,000
Immunofluorescence: 1:50-1:500
Immunohistochemistry (paraffin): 1:50-1:500
Immunohistochemistry (frozen): 1:50-1:500

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Polyclonal antibody supplied in PBS, 20% glycerol and 0.05% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.
The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20 °C for long term storage. Store at 2-8 °C for up to one month.

References for anti-mCherry antibody (ABIN1440058)

Wong, Selten, Rosés-Novella, Sreenivasan, Pallas-Bazarra, Serafeimidou-Pouliou, Hanusz-Godoy, Oozeer, Edwards, Marín: "Serotonergic regulation of bipolar cell survival in the developing cerebral cortex." in: Cell reports, Vol. 40, Issue 1, pp. 111037, (2022) (PubMed).

Yoon, Piguel, Khalatyan, Dionisio, Savas, Penzes: "Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome." in: Molecular psychiatry, (2021) (PubMed).

McCallum, Obata, Fourli, Boeing, Peddie, Xu, Horswell, Kelsh, Collinson, Wilkinson, Pin, Pachnis, Heanue: "Enteric glia as a source of neural progenitors in adult zebrafish." in: eLife, Vol. 9, (2020) (PubMed).

Llorca, Ciceri, Beattie, Wong, Diana, Serafeimidou-Pouliou, Fernández-Otero, Streicher, Arnold, Meyer, Hippenmeyer, Maravall, Marin: "A stochastic framework of neurogenesis underlies the assembly of neocortical cytoarchitecture." in: eLife, Vol. 8, (2020) (PubMed).

Miesfeld, Glaser, Brown: "The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody." in: Gene expression patterns : GEP, Vol. 27, pp. 114-121, (2018) (PubMed).

Oliveira, Lemaitre, Murawala, Tazaki, Drechsel, Tanaka: "Pseudotyped baculovirus is an effective gene expression tool for studying molecular function during axolotl limb regeneration." in: Developmental biology, Vol. 433, Issue 2, pp. 262-275, (2018) (PubMed).

Lin, Coleman, Peterson, Zunitch, Jang, Herrick, Schwob: "Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency." in: Cell stem cell, Vol. 21, Issue 6, pp. 761-774.e5, (2018) (PubMed).

Rocca, Goodman, Dulin, Haquang, Gertsman, Blondelle, Smith, Heyser, Cherqui: "Transplantation of wild-type mouse hematopoietic stem and progenitor cells ameliorates deficits in a mouse model of Friedreich's ataxia." in: Science translational medicine, Vol. 9, Issue 413, (2018) (PubMed).

Saito, Otsu, Hsu, Chuang, Yanagisawa, Shieh, Kaitsuka, Wei, Tomizawa, Sung: "Tctex-1 controls ciliary resorption by regulating branched actin polymerization and endocytosis." in: EMBO reports, Vol. 18, Issue 8, pp. 1460-1472, (2018) (PubMed).

Shevelkin, Terrillion, Abazyan, Kajstura, Jouroukhin, Rudow, Troncoso, Linden, Pletnikov: "Expression of mutant DISC1 in Purkinje cells increases their spontaneous activity and impairs cognitive and social behaviors in mice." in: Neurobiology of disease, Vol. 103, pp. 144-153, (2018) (PubMed).

Kodani, Soya, Sakurai: "Excitation of GABAergic Neurons in the Bed Nucleus of the Stria Terminalis Triggers Immediate Transition from Non-Rapid Eye Movement Sleep to Wakefulness in Mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 30, pp. 7164-7176, (2017) (PubMed).

Castellano, Mosher, Abbey, McBride, James, Berdnik, Shen, Zou, Xie, Tingle, Hinkson, Angst, Wyss-Coray: "Human umbilical cord plasma proteins revitalize hippocampal function in aged mice." in: Nature, Vol. 544, Issue 7651, pp. 488-492, (2017) (PubMed).

Agarwal, Wu, Hughes, Fukaya, Tischfield, Langseth, Wirtz, Bergles: "Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes." in: Neuron, Vol. 93, Issue 3, pp. 587-605.e7, (2017) (PubMed).

Abraira, Kuehn, Chirila, Springel, Toliver, Zimmerman, Orefice, Boyle, Bai, Song, Bashista, ONeill, Zhuo, Tsan, Hoynoski, Rutlin, Kus, Niederkofler, Watanabe, Dymecki, Nelson, Heintz, Hughes, Ginty: "The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn." in: Cell, Vol. 168, Issue 1-2, pp. 295-310.e19, (2017) (PubMed).

Krishnamurthy, Khamo, Mei, Turgeon, Ashraf, Mondal, Patel, Risner, Cho, Yang, Zhang: "Reversible optogenetic control of kinase activity during differentiation and embryonic development." in: Development (Cambridge, England), Vol. 143, Issue 21, pp. 4085-4094, (2017) (PubMed).

Falkner, Grade, Dimou, Conzelmann, Bonhoeffer, Götz, Hübener: "Transplanted embryonic neurons integrate into adult neocortical circuits." in: Nature, Vol. 539, Issue 7628, pp. 248-253, (2017) (PubMed).

Vyas, Wu, Zimmerman, Fuchs, Glowatzki: "Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice." in: Journal of the Association for Research in Otolaryngology : JARO, Vol. 18, Issue 1, pp. 139-151, (2017) (PubMed).

Sargiannidou, Kim, Kyriakoudi, Eun, Kleopa: "A start codon CMT1X mutation associated with transient encephalomyelitis causes complete loss of Cx32." in: Neurogenetics, Vol. 16, Issue 3, pp. 193-200, (2016) (PubMed).

Merienne, Delzor, Viret, Dufour, Rey, Hantraye, Déglon: "Gene transfer engineering for astrocyte-specific silencing in the CNS." in: Gene therapy, Vol. 22, Issue 10, pp. 830-9, (2016) (PubMed).

Target Details for mCherry

Target: mCherry (mCherry Fluorescent Protein)

Alternative Name: mCherry

Background: Synonyms: Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato