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Vimentin antibody (pSer33)

The Mouse Monoclonal anti-Vimentin antibody has been validated for WB and IF. It is suitable to detect Vimentin in samples from Human.
Catalog No. ABIN1449152

Quick Overview for Vimentin antibody (pSer33) (ABIN1449152)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 348
  • 129
  • 127
  • 63
  • 61
  • 49
  • 43
  • 38
  • 31
  • 22
  • 21
  • 13
  • 13
  • 8
  • 8
  • 6
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 183
  • 153
  • 10
  • 6
  • 6
  • 5
  • 2
  • 2
Mouse

Clonality

  • 184
  • 182
Monoclonal

Conjugate

  • 215
  • 20
  • 13
  • 12
  • 9
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
This Vimentin antibody is un-conjugated

Application

  • 306
  • 152
  • 117
  • 100
  • 91
  • 61
  • 57
  • 40
  • 39
  • 35
  • 28
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  • 22
  • 8
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  • 2
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF)

Clone

YT33
  • Binding Specificity

    • 32
    • 31
    • 17
    • 16
    • 11
    • 10
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer33

    Sequence

    CSYVTTpSTR TYS

    Specificity

    This antibody recognizes the site-specific phosphorylation of vimentin at Ser33.

    Cross-Reactivity (Details)

    Species reactivity (tested):Human

    Purification

    Protein A agarose

    Immunogen

    KLH conjugated phospho-peptide PV33

    Isotype

    IgG1
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Buffer

    PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

    Preservative

    Without preservative

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence.Synonyms: VIM

    Gene ID

    7431

    NCBI Accession

    NP_003371

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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