CYBA antibody

Catalog No. ABIN1449193

This Mouse Monoclonal antibody specifically detects CYBA in FACS. It exhibits reactivity toward Human.

Quick Overview for CYBA antibody (ABIN1449193)

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYBA antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 7D5

Product Details anti-CYBA Antibody

Specificity: This antibody reacts with an extracellular epitope of gp91phox. The antibody recognizes the epitope in the native conformation or after treatment with guanidine or urea, but SDS treatment will abolish antibody binding.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A agarose

Immunogen: Human cytochrome b rich fraction

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliqouts) at -20 °C.

Target Details for CYBA

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Alternative Name: Cytochrome B-245 Light Chain

Background: The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2 -), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome B558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2 -. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.Synonyms: CYBA, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox

Gene ID: 1535

NCBI Accession: NP_000092

UniProt: P13498

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis