CYBA antibody (FITC)

Catalog No. ABIN1449194

The Mouse Monoclonal anti-CYBA antibody has been validated for FACS. It is suitable to detect CYBA in samples from Human.

Quick Overview for CYBA antibody (FITC) (ABIN1449194)

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYBA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 7D5

Product Details anti-CYBA Antibody (FITC)

Specificity: This antibody reacts with an extracellular epitope of gp91phox. The antibody recognizes the epitope in the native conformation. This antibody reacts with Flavocytochrome b558 on flow cytometry.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A agarose

Immunogen: Human cytochrome b rich fraction

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, 0.09 % NaN3, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for CYBA

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Alternative Name: Cytochrome B-245 Light Chain

Background: The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2 -), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2 -. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.Synonyms: CYBA, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox

Gene ID: 1535

NCBI Accession: NP_000092

UniProt: P13498

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis