14-3-3 gamma antibody (N-Term)

Catalog No. ABIN1449274

This Mouse Monoclonal antibody specifically detects 14-3-3 gamma in WB and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for 14-3-3 gamma antibody (N-Term) (ABIN1449274)

Target: 14-3-3 gamma (YWHAG1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: KC21

Product Details anti-14-3-3 gamma Antibody

Binding Specificity: AA 1-12, N-Term

Specificity: This antibody reacts with 14-3-3γ, which the N-terminal Met is removed as a result of acetylated the N-terminal Val, on Western blotting. This antibody does not recognize unprocessed (non-modified) 14-3-3γ.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Protein A agarose

Immunogen: KLH-conjugated human 14-3-3gamma N-terminal peptides (1-12 aa)

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliqouts) at -20 °C.

Target Details for 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Alternative Name: 14-3-3 Protein gamma

Background: The 14-3-3 protein family comprises critical regulatory molecules involved in signaling during cell division, proliferation, and apoptosis. 14-3-3 γ, a subtype of the 14-3-3 family of proteins, was thought to be brain- and neuron-specific. However, 14-3-3 γ was not neuron-specific but also expressed in astrocytes. Endogenous 14-3-3 γ proteins in immature astrocytes appeared filamentous and co-localized with filamentous actin (F-actin). And, 14-3-3 γ proteins play a role in cytoskeletal function during the process of cell division and apoptosis in astrocytes in association with actin. This expression is induced in arterial trauma by cytokines, and suggests that this protein may play an important role in progression of vascular proliferative diseases.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAG

Molecular Weight: 33 kDa

Gene ID: 7532

NCBI Accession: NP_036611

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase