HMGB1 antibody (Internal Region)

Catalog No. ABIN1449284

The Mouse Monoclonal anti-HMGB1 antibody has been validated for WB. It is suitable to detect HMGB1 in samples from Human, Mouse and Rat.

Quick Overview for HMGB1 antibody (Internal Region) (ABIN1449284)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 4C9

Product Details anti-HMGB1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts with HMGB1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Protein A agarose

Immunogen: Synthetic peptide corresponding to internal region of human HMGB1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliqouts) at -20 °C.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: High mobility group box 1 (HMGB1), named for its rapid migration properties on electrophoretic gels, is a member of the nonhistone chromatin-associated proteins. HMGB1 is translated as a 214 amino acid protein, and extensively modified posttranslationally, by glycosylation, acylation, methylation, and phosphorylation. The primary structure is evolutionarily conserved, with 100 % amino acid sequence homology between rat and mouse, and 99 % homology between rodent and human. Intracellular HMGB1 has been studied previously for its roles in binding DNA, stabilizing nucleosome formation, as a general transcription factor for nucleolar and mitochondrial RNA polymerases, and as a gene- and tissue-specific transcriptional regulator that can enhance transcription and/or replication. Extracellular HMGB1 is recently implicated as a late mediator of delayed endotoxin lethality, because murine and human macrophages/monocytes release large amounts of a 29 kDa form of HMGB1 when stimulated by exposure to bacterial endotoxin.Synonyms: Amphoterin, HMG-1, HMG1, HMGB-1, High mobility group protein 1, High mobility group protein B1

Gene ID: 3146

NCBI Accession: NP_002119

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome