ATG12 antibody (N-Term)
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- Target See all ATG12 Antibodies
- ATG12 (Autophagy Related 12 (ATG12))
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Binding Specificity
- AA 44-73, N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATG12 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody recognizes Human APG12L (N-term). Other species not tested.
- Purification
- Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
- Immunogen
- KLH conjugated synthetic peptide between 44~73 amino acids from the N-term of Human APG12L Genename: ATG12
- Isotype
- Ig Fraction
- Top Product
- Discover our top product ATG12 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS containing 0.09 % (W/V) Sodium Azide as preservative
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- ATG12 (Autophagy Related 12 (ATG12))
- Alternative Name
- ATG12 / APG12 (ATG12 Products)
- Background
- Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12
- Molecular Weight
- 15113 Da
- Gene ID
- 9140
- NCBI Accession
- NP_004698
- Pathways
- Autophagy
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