SCUBE3 antibody (Middle Region)

Catalog No. ABIN1449994

This Rabbit Polyclonal antibody specifically detects SCUBE3 in WB, IF and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for SCUBE3 antibody (Middle Region) (ABIN1449994)

Target: SCUBE3 (Signal Peptide, CUB Domain, EGF-Like 3 (SCUBE3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCUBE3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-SCUBE3 Antibody

Binding Specificity: Middle Region

Purification: Affinity chromatography purified via peptide column

Immunogen: A 15 amino acid synthetic peptide near the center of Human SCUBE3

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SCUBE3

Target: SCUBE3 (Signal Peptide, CUB Domain, EGF-Like 3 (SCUBE3))

Alternative Name: SCUBE3

Background: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart. Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth.. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.Synonyms: CEGF3, Signal peptide CUB and EGF-like domain-containing protein 3

Molecular Weight: 109 kDa

Gene ID: 222663

NCBI Accession: NP_689966