DDX60 antibody (Middle Region)

Catalog No. ABIN1450010

The Rabbit Polyclonal anti-DDX60 antibody is suitable to detect DDX60 in samples from Human, Mouse and Rat. It has been validated for WB, IF and EIA.

Quick Overview for DDX60 antibody (Middle Region) (ABIN1450010)

Target: DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX60 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-DDX60 Antibody

Binding Specificity: Middle Region

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity chromatography purified via peptide column

Immunogen: 17 amino acid synthetic peptide near the center of Human DDX60

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DDX60

Target: DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))

Alternative Name: DDX60

Background: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.Synonyms: FLJ20035, Probable ATP-dependent RNA helicase DDX60

Molecular Weight: 188 kDa

Gene ID: 55601

NCBI Accession: NP_060101