SPARC antibody

Catalog No. ABIN1450173

The Mouse Monoclonal anti-SPARC antibody (Clone N50) (ABIN1450173) specifically detects SPARC in WB, FACS, IHC (p), IF, IHC (fro), EIA and RIA. The antibody is reactive with Human, Rat and Cow samples.

Quick Overview for SPARC antibody (ABIN1450173)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Clone: N50

Product Details anti-SPARC Antibody

Specificity: Recognizes Human and Bovine bone Osteonectin, Human platelet Osteonectin. SPARC produced by various cells grown in culture. Cross reacts with Rat Osteonectin.Aff. Const.:5 x 10e-9 M

Purification: Ammonium Sulfate Precipitation, Ion Exchange Chromatography and Gel Filtration.

Immunogen: Crude bone extract containing native Osteonectin.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 10.8 mg/mL (OD280 nm, E1% = 14)

Buffer: 50 % Glycerol/H2O (vol/vol), None

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC / Osteonectin

Background: SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression.Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

Gene ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy