mLC-2 (AA 5-54), (pThr17) antibody

Catalog No. ABIN1450272

Product Details

Target: mLC-2

Binding Specificity: AA 5-54, pThr17

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Specificity:

MLC-2 (Phospho-Thr17+Ser18) Antibody detects endogenous levels of MLC-2 only when phosphorylated at Thr17 and Ser18.

PhosphorylationH:T17+S18, M:T19+S20, R:T18+S19

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Immunogen: The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

References

Huang, Cheng, Su, Lin: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, (2015) (PubMed).

Target Details

Target: mLC-2

Background:

Synonyms: MLCB, MLRA, MLRM, MRCL3, Myosin RLC, Myosin RLC-A, Myosin regulatory light chain, Myosin regulatory light chain 2, nonsarcomeric, Myosin

NCBI Gene Symbol: MYL12A

Molecular Weight: 19 kDa

Gene ID: 10627

OMIM: 609211

UniProt: P19105, O14950, P24844