mLC-2 (pThr17) antibody

Catalog No. ABIN7219090

The Rabbit Polyclonal anti-mLC-2 antibody (ABIN7219090) specifically detects mLC-2 in IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for mLC-2 (pThr17) antibody (ABIN7219090)

Target: mLC-2

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details

Binding Specificity: pThr17

Purpose: Rabbit Anti-MLC-2 (phospho Thr17/S18) Polyclonal Antibody

Specificity: Phospho-MLC-2 (T17/S18) Polyclonal Antibody detects endogenous levels of MLC-2 protein only when phosphorylated at T17/S18.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MLC-2 Phospho-Thr17/S18

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: mLC-2

Background: MYL12A, MLCB, MRLC3, RLC, Myosin regulatory light chain 12A, MLC-2B, Myosin RLC, Myosin regulatory light chain 2, nonsarcomeric, Myosin regulatory light chain MRLC3, MYL12B, MRLC2, MYLC2B, Myosin regulatory light chain 12B, MLC-2A, MLC-2, MMYL12A encodes a nonsarcomeric myosin regulatory light chain. Myosin regulatory light chain 12A is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.

Gene ID: 10627

UniProt: P19105, O14950, P24844