ITK antibody (pTyr512)

Catalog No. ABIN1450332

This anti-ITK antibody is a Rabbit Polyclonal antibody detecting ITK in ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for ITK antibody (pTyr512) (ABIN1450332)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-ITK Antibody

Binding Specificity: AA 478-527, pTyr512

Specificity:

ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512.

PhosphorylationH:Y512, M:Y517

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Immunogen: The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK

Background:

Synonyms: EMT, IL-2-inducible T-cell kinase, IL2-inducible T-cell kinase, ITK/TSK, Kinase EMT, kinase Itk, LYK, Lyk, T-cell-specific kinase, TLK, TSK

NCBI Gene Symbol: ITK

Molecular Weight: 71 kDa

Gene ID: 3702

OMIM: 186973

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway