HCLS1 antibody (pTyr397)

Catalog No. ABIN1450515

The Rabbit Polyclonal anti-HCLS1 antibody (ABIN1450515) specifically detects HCLS1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HCLS1 antibody (pTyr397) (ABIN1450515)

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCLS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HCLS1 Antibody

Binding Specificity: AA 366-415, pTyr397

Purpose: Lck BP-1 (phospho Tyr397) Antibody

Specificity: Phospho-Lck BP-1 (Y397) Polyclonal Antibody detects endogenous levels of Lck BP-1 protein only when phosphorylated at Y397.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human HS1 around the phosphorylation site of Tyr397. AA range:366-415

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HCLS1

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Alternative Name: HS1

Background:

Synonyms: HCLS1, HS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75

Background: developmental stage:Expressed in early stage of myeloid and erythroid differentiation.,function:Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.,PTM:Phosphorylated by LYN, rapidly after cross-linking of surface IgM on B-cells.,similarity:Contains 1 SH3 domain.,similarity:Contains 4 cortactin repeats.,subunit:Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3.,tissue specificity:Expressed only in tissues and cells of hematopoietic origin.,

Gene Name: HCLS1

Protein Name: Hematopoietic lineage cell-specific protein

Molecular Weight: 53 kDa

Gene ID: 3059

OMIM: 601306

UniProt: P14317

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Maintenance of Protein Location