GIT1 antibody (AA 561-610)

Catalog No. ABIN1450893

The Rabbit Polyclonal anti-GIT1 antibody (ABIN1450893) specifically detects GIT1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for GIT1 antibody (AA 561-610) (ABIN1450893)

Target: GIT1

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-GIT1 Antibody

Binding Specificity: AA 561-610

Purpose: GIT1 Antibody

Specificity: GIT1 Polyclonal Antibody detects endogenous levels of GIT1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human GIT1. AA range:561-610

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1

Background:

Synonyms: GIT1, ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1

Background: domain:The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.,function:GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.,PTM:Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 3 ANK repeats.,subcellular location:Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.,subunit:Interacts with G protein-coupled receptor kinases: ADRBK1/GRK2, PPFIA1 and PPFIA4. Interacts with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK (By similarity). Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1.,

Gene Name: GIT1

Protein Name: ARF GTPase-activating protein GIT1

Molecular Weight: 84 kDa

Gene ID: 28964

OMIM: 608434

UniProt: Q9Y2X7