FLIP antibody (AA 181-230)

Catalog No. ABIN1450919

The Rabbit Polyclonal anti-FLIP antibody (ABIN1450919) specifically detects FLIP in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FLIP antibody (AA 181-230) (ABIN1450919)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FLIP Antibody

Binding Specificity: AA 181-230

Purpose: c-FLIP Antibody

Specificity: C-FLIP Polyclonal Antibody detects endogenous levels of c-FLIP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human in ternal CFLAR. AA range:181-230

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR

Background:

Synonyms: CFLAR, CASH, CASP8AP1, CLARP, MRIT, CASP8 and FADD-like apoptosis regulator, Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FAD

Background: The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011],

Gene Name: CFLAR

Protein Name: CASP8 and FADD-like apoptosis regulator

Molecular Weight: 55 kDa

Gene ID: 8837

OMIM: 603599

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development