ORC1 antibody (AA 331-380)

Catalog No. ABIN1450965

The Rabbit Polyclonal anti-ORC1 antibody (ABIN1450965) specifically detects ORC1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ORC1 antibody (AA 331-380) (ABIN1450965)

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ORC1 Antibody

Binding Specificity: AA 331-380

Purpose: ORC1 Antibody

Specificity: ORC1 Polyclonal Antibody detects endogenous levels of ORC1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human ORC1L. AA range:331-380

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ORC1

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Alternative Name: ORC1L

Background:

Synonyms: ORC1, ORC1L, PARC1, Origin recognition complex subunit 1, Replication control protein 1

Background: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins.The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcr

Gene Name: ORC1

Protein Name: Origin recognition complex subunit 1

Molecular Weight: 97 kDa

Gene ID: 4998

OMIM: 601902

UniProt: Q13415

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA